N-Acetyl--glucosamine-specific lectin purified from Vibrio cholerae 01

作者：Dipti Sasmal, Bhakti Guhathakurta, Amit Ghosh, Chiranjib Pal, Abhirup Datta · 发表于：FEMS Microbiology Letters · 年份：1992 · 被引用次数：2 · 研究领域：Vibrio bacteria research studies、Escherichia coli research studies、Bacteriophages and microbial interactions

An N-acetyl-D-glucosamine-specific cell associated hemagglutinin (HA) was isolated and purified from a strain of Vibrio cholerae 01 by chitin affinity chromatography followed by separation on Bio Gel P-150. A single stained protein band of 47 kDa in sodium dodecyl sulfate-polyacryl-amide gel electrophoresis (SDS-PAGE) was observed with the purified HA. HA-antisera produced a single precipitin band against the purified HA in an immunodiffusion test without exhibiting any reactivity towards purified lipopolysaccharide (LPS). Purified HA, used as solid-phase antigen in an enzyme-linked immunosorbent assay (ELISA), reacted strongly with HA-antisera but cross-reacted negligibly with antisera raised against purified LPS. Hemagglutinating activity of the purified HA was highly sensitive to N-acetyl-D-glucosamine. The immunogold-labelling method using HA-antisera confirmed the location of the HA on the surface of the bacterial cells. The HA-antisera reacted with