H19 non-coding RNA in urine cells detects urothelial carcinoma: a pilot study

作者：Ilan Gielchinsky, Michal Gilon, Rasha Abu-lail, Imad Matouk, Avraham Hochberg, Ofer Nathan Gofrit, Iddo Z. Ben‐Dov · 发表于：Biomarkers · 年份：2017 · DOI：10.1080/1354750x.2016.1276625 · 被引用次数：14 · 研究领域：Cancer-related molecular mechanisms research、Molecular Biology Techniques and Applications、Bladder and Urothelial Cancer Treatments

CONTEXT: Urothelial carcinoma (UC) is common and highly recurrent. Diagnosis and follow-up involve invasive cystoscopies. OBJECTIVE: To evaluate H19 RNA in urine cells as diagnostic tool for UC. MATERIALS AND METHODS: RT-PCR analysis of urine samples from healthy volunteers and UC patients. RESULTS: H19 RNA was unequivocally detected in the urine of 90.5% of patients and 25.9% of controls. H19 copies were three orders of magnitude higher in patients. Receiver operating characteristic analysis showed an area under the curve of 0.933. CONCLUSIONS: This pilot study shows that urinary cell H19 is a highly sensitive test for UC and pending verification could transform patient management.