A Gold Nanoparticle-enhanced Surface Plasmon Resonance Aptasensor for the Detection of 2,4,6-trinitrotoluene

作者：Hongxia Hao, Ruiqin Yang, Junpeng Tan, Bin Hao, Chuo Wang, Yifei Ren · 发表于：Journal of Forensic Science and Medicine · 年份：2016 · DOI：10.4103/2349-5014.197934 · 被引用次数：8 · 研究领域：Advanced biosensing and bioanalysis techniques、Plasmonic and Surface Plasmon Research、Luminescence and Fluorescent Materials

A gold nanoparticle-enhanced surface plasmon resonance (SPR) aptasensor was developed for high-specificity and high-sensitivity detection of 2,4,6-trinitrotoluene (TNT). Self-assembly film-forming technology was used to modify the gold surface of the sensor chip with 2,4,6-trinitrophenyl glycine, a TNT analogue, using polyethylene glycol to which the thiol group and carboxyl group are attached. Aptamer-gold nanoparticle complexes were formed through Au-S bonding. To detect TNT, the samples were incubated with the aptamer-gold nanoparticle complexes, and the solution competition method was applied through the SPR aptasensor. The results showed that the SPR aptasensor achieved fast, real-time detection of TNT. This gold nanoparticle-enhanced SPR aptasensor is suitable for TNT detection in the field of public safety and environmental monitoring.