Identification and Salinity Tolerance of the Western Hemisphere MusselMytella charruana(D'Orbigny, 1842) in the Philippines

作者：Michael A Rice, Paul D. Rawson, Ashley D. Salinas, Westly R. Rosario · 发表于：Journal of Shellfish Research · 年份：2016 · DOI：10.2983/035.035.0415 · 被引用次数：43 · 研究领域：Marine Bivalve and Aquaculture Studies、Marine Biology and Ecology Research、Ocean Acidification Effects and Responses

Beginning in 2014, mussels have been found by shellfishers in parts of the Philippines that are morphologically distinct from native mytilids. These mussels, with a thick black periostracum, were first found in Manila Bay near an international shipping port, and later in 2014 appeared in western Tambac Bay (approximately 16.28° N, 119.9° E). The next year (July 2015), they were found near the village of Tucok (Dagupan City; 16.0272° N, 120.3147° E), and more recently (early 2016) they have been observed in Longos, San Fabian, Pangasinan (16.1887, 120.4043). About 50 mussels from Tucok were preserved in 95% ethanol and sent to the University of Maine for genetic evaluation. Sequencing of mitochondrial cytochrome oxidase I polymerase chain reaction products identified the nonnative mussels as Mytella charruana, the charru mussel, native to the tropical Caribbean and western Pacific coasts of South America. Further analysis indicates that populations on the Caribbean Coast of South America are the likely source of the Philippine charru mussels. Two salinity tolerance experiments were also conducted; in the first experiment, Philippine charru mussels were conditioned at salinity 5 (similar to the salinity of the collection site) for 7 days, then subjected to a salinity shock by directly transferring them to different salinity levels (10, 15, 20, 25, 30, and 35) for 12 days. In the second experiment, replicate groups of mussels were conditioned in 30 salinity, then for each replic...