The Heterogeneity of Ly6Chi Monocytes Controls Their Differentiation into iNOS+ Macrophages or Monocyte-Derived Dendritic Cells

作者：Shinelle Menezes, Daisy Melandri, Giorgio Anselmi, Thibaut Perchet, Jakob Loschko, Juan Dubrot, Rajen Patel, Emmanuel L. Gautier, Stéphanie Hugues, M. Paula Longhi, Jake Y. Henry, Sergio A. Quezada, Grégoire Lauvau, Ana‐Maria Lennon‐Duménil, Enrique Gutiérrez-Martínez, Alain Bessis, Elisa Gomez Perdiguero, Christian E. Jacome-Galarza, Hannah Garner, Frédéric Geissmann, Rachel Golub, Michel C. Nussenzweig, Pierre Guermonprez · 发表于：Immunity · 年份：2016 · DOI：10.1016/j.immuni.2016.12.001 · 被引用次数：291 · 研究领域：Immune cells in cancer、Immunotherapy and Immune Responses、Immune Cell Function and Interaction

Inflammation triggers the differentiation of Ly6C hi monocytes into microbicidal macrophages or monocyte-derived dendritic cells (moDCs). Yet, it is unclear whether environmental inflammatory cues control the polarization of monocytes toward each of these fates or whether specialized monocyte progenitor subsets exist before inflammation. Here, we have shown that naive monocytes are phenotypically heterogeneous and contain an NR4A1- and Flt3L-independent, CCR2-dependent, Flt3 + CD11c − MHCII + PU.1 hi subset. This subset acted as a precursor for FcγRIII + PD-L2 + CD209a + , GM-CSF-dependent moDCs but was distal from the DC lineage, as shown by fate-mapping experiments using Zbtb46 . By contrast, Flt3 − CD11c − MHCII − PU.1 lo monocytes differentiated into FcγRIII + PD-L2 − CD209a − iNOS + macrophages upon microbial stimulation. Importantly, Sfpi1 haploinsufficiency genetically distinguished the precursor activities of monocytes toward moDCs or microbicidal macrophages. Indeed, Sfpi1 +/− mice had reduced Flt3 + CD11c − MHCII + monocytes and GM-CSF-dependent FcγRIII + PD-L2 + CD209a + moDCs but generated iNOS + macrophages more efficiently. Therefore, intercellular disparities of PU.1 expression within naive monocytes segregate progenitor activity for inflammatory iNOS + macrophages or moDCs.