Use of polyvinylpyrrolidone-iodine solution for sterilisation and preservation improves mechanical properties and osteogenesis of allografts

作者：Yantao Zhao, Xiantong Hu, Zhonghai Li, Fuli Wang, Yang Xia, Shuxun Hou, Hongbin Zhong, Feimin Zhang, Ning Gu · 发表于：Scientific Reports · 年份：2016 · DOI：10.1038/srep38669 · 被引用次数：15 · 研究领域：Tissue Engineering and Regenerative Medicine、Infectious Aortic and Vascular Conditions、Orthopaedic implants and arthroplasty

Abstract Allografts eliminate the disadvantages associated with autografts and synthetic scaffolds but are associated with a disease-transmission risk. Therefore, allograft sterilisation is crucial. We aimed to determine whether polyvinylpyrrolidone-iodine (PVP-I) can be used for sterilisation and as a new wet-preservation method. PVP-I–sterilised and preserved allografts demonstrated improved mechanical property, osteogenesis, and excellent microbial inhibition. A thigh muscle pouch model of nude mice showed that PVP-I–preserved allografts demonstrated better ectopic formation than Co 60 -sterilised allografts (control) in vivo (P < 0.05). Furthermore, the PVP-I–preserved group showed no difference between 24 h and 12 weeks of allograft preservation (P > 0.05). PVP-I–preserved allografts showed more hydrophilic surfaces and PVP-I–sterilised tendons showed higher mechanical strength than Co 60 -sterilised tendons (P < 0.05). The level of residual PVP-I was higher without washing and with prolonged preservation (P < 0.05). In vitro cellular tests showed that appropriate PVP-I concentration was nontoxic to preosteoblast cells, and cellular differentiation measured by alkaline phosphatase activity and osteogenic gene markers was enhanced (P < 0.05). Therefore, the improved biological performance of implanted allografts may be attributable to better surface properties and residual PVP-I, and PVP-I immersion can be a simple, easy method for allograft sterilisation a...