Anti-inflammatory and neuroprotective effects of sanguinarine following cerebral ischemia in rats

作者：Qin Wang, Peng Dai, Han Bao, Ping Liang, Wei Wang, An Xing, Jianbin Sun · 发表于：Experimental and Therapeutic Medicine · 年份：2016 · DOI：10.3892/etm.2016.3947 · 被引用次数：55 · 研究领域：MicroRNA in disease regulation、Berberine and alkaloids research、Neuroinflammation and Neurodegeneration Mechanisms

Stroke is one of the leading causes of mortality worldwide. Protective agents that can diminish injuries caused by cerebral ischemia-reperfusion (I/R) are important in alleviating the harmful outcomes of stroke. The aim of the present study was to investigate the protective role of sanguinarine in cerebral I/R injury. A rat middle cerebral artery occlusion model was used to assess the clinical effect of sanguinarine, and inflammatory cytokines in the serum were detected by ELISA. Western blotting was performed to examine the change in levels of apoptosis‑associated proteins in the injured brains. The results suggested that sanguinarine, an anti‑inflammatory agent derived from the roots of Sanguinaria canadensis, improved the state of cerebral ischemia in a rat model. The data demonstrated that when rats were treated with sanguinarine prior to middle cerebral artery occlusion, the infarct volume was reduced significantly. The inflammatory factors tumor necrosis factor‑α, interleukin (IL)‑6 and IL‑1β were measured in sanguinarine and vehicle‑treated groups using an enzyme‑linked immunosorbent assay, and the expression levels of the three factors were significantly reduced following treatment with sanguinarine (P<0.05). In addition, western blot analysis demonstrated that the ratio of B‑cell lymphoma 2/Bcl-2-associated X protein was significantly increased following treatment with sanguinarine (P<0.05). The study demonstrated that sanguinarine exerts a protective effect in cereb...