Mechanisms of transcriptional activation of the stimulator of interferon genes by transcription factors CREB and c-Myc

作者：Yanyan Wang, Rui Jin, Guo‐Ping Zhou, Hua‐Guo Xu · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.13183 · 被引用次数：22 · 研究领域：interferon and immune responses、Cytokine Signaling Pathways and Interactions、RNA regulation and disease

// Yan-Yan Wang 1, 2, 3 , Rui Jin 2 , Guo-Ping Zhou 2 , Hua-Guo Xu 1 1 Department of Laboratory Medicine, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China 2 Department of Pediatrics, The First Affiliated Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210029, China 3 Department of Pediatrics, Nanjing First Hospital, Nanjing Medical University, Nanjing, Jiangsu Province 210006, China Correspondence to: Hua-Guo Xu, email: huaguoxu@njmu.edu.cn Guo-Ping Zhou, email: gpzhou2003@126.com Keywords: stimulator of interferon genes, promoter, CREB, c-Myc Received: September 09, 2016 Accepted: October 14, 2016 Published: November 07, 2016 ABSTRACT Stimulator of interferon genes (STING) plays an important role in host defense, autoimmune disease, osteoclast differentiation and anti-tumor response. Although many downstream targets have been studied in depth, the regulation of STING gene expression remains largely unknown. Here we demonstrate that transcription factors CREB and c-Myc maintain the transcriptional activity of STING. By 5′-rapid amplification of cDNA ends analysis, we identified the transcriptional start site (TSS) of STING. We illustrated that the region -124/+1 relative to TSS was sufficient for full promoter activity by a series of 5′ deletion promoter constructs. Transcriptional activity of the STING minimal promoter was dependent on CREB and c-Myc binding motifs and was abolished after mutation of these two...