A regulatory loop involving miR-29c and Sp1 elevates the TGF-β1 mediated epithelial-to-mesenchymal transition in lung cancer

作者：Hai-wei Zhang, Enwen Wang, Lixian Li, Shouhui Yi, Luchun Li, Faliang Xu, Donglin Wang, Yongzhong Wu, Weiqi Nian · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.13137 · 被引用次数：58 · 研究领域：Cancer-related molecular mechanisms research、MicroRNA in disease regulation、Cancer Cells and Metastasis

// Hai-wei Zhang 1, 2 , En-wen Wang 3 , Li-xian Li 1, 2 , Shou-hui Yi 1, 2 , Lu-chun Li 3 , Fa-liang Xu 4 , Dong-lin Wang 3 , Yong-zhong Wu 5 , Wei-qi Nian 1, 2 1 Key Laboratory of Oncology, Chongqing Cancer Institute, Chongqing Cancer Hospital, Chongqing Cancer Center, Chongqing, China 2 Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing Cancer Institute, Chongqing, China 3 Department of Oncology, Chongqing Cancer Institute, Chongqing, China 4 Center of Breast Cancer, Chongqing Cancer Institute, Chongqing, China 5 Department of Radiotherapy, Chongqing Cancer Institute, Chongqing, China Correspondence to: Wei-qi Nian, email: nwqone@126.com Keywords: miR-29c, metastasis, TGF-β1, sp1, EMT Received: March 27, 2016      Accepted: October 31, 2016      Published: November 05, 2016 ABSTRACT Specificity protein1 (Sp1) is required for TGF-β-induced epithelial-to-mesenchymal transition (EMT) which has been demonstrated to aggravate the progression of cancer including lung cancer. microRNA-29c (miR-29c) is identified to inhibit EMT, but the correlation between miR-29c and Sp1 in human lung cancer remain incompletely clarified. Here, we confirmed decreased expression of miR-29c and enhanced expression of Sp1 in lung cancer tissues ( n = 20) and found that Sp1 could be targeted and inhibited by miR-29c. Besides, the expression of miR-29c was down-regulated in high-met...