Alkene hydrogenation activity of enoate reductases for an environmentally benign biosynthesis of adipic acid

作者：Jeong Chan Joo, Anna N. Khusnutdinova, Robert Flick, Taeho Kim, Uwe T. Bornscheuer, Alexander F. Yakunin, Radhakrishnan Mahadevan · 发表于：Chemical Science · 年份：2016 · DOI：10.1039/c6sc02842j · 被引用次数：99 · 研究领域：Enzyme Catalysis and Immobilization、Microbial Metabolic Engineering and Bioproduction、Biofuel production and bioconversion

. Purified ERs exhibit a broad substrate spectrum including aromatic and aliphatic 2-enoates and a significant oxygen tolerance. The discovery of the hydrogenation activity of ERs contributes to an understanding of the catalytic mechanism of these poorly characterized enzymes and enables the environmentally benign biosynthesis of adipic acid and other chemicals from renewable resources.