The transcription factor cyclic adenosine 3′,5′‐monophosphate response element‐binding protein enhances the odonto/osteogenic differentiation of stem cells from the apical papilla

作者：Shaoqiang Su, Youyu Zhu, Shaopeng Li, Yan Liang, J. Zhang · 发表于：International Endodontic Journal · 年份：2016 · DOI：10.1111/iej.12709 · 被引用次数：8 · 研究领域：Bone and Dental Protein Studies、dental development and anomalies、Endodontics and Root Canal Treatments

AIM: To investigate the role of cAMP response element-binding protein (CREB) in the regulation of odonto/osteogenic differentiation of stem cells from the apical papilla (SCAPs). METHODOLOGY: Stem cells from the apical papilla were obtained from human impacted third molars (n = 15). Isolated SCAPs were transfected with CREB overexpressing/silenced lentivirus. Transfected cells were stained with alizarin red to investigate mineralized nodule formation. The expression of the mineralization-related genes, alkaline phosphatase (ALP), collagen type I (Col I), runt-related transcription factor 2 (RUNX2), osterix (OSX) and osteocalcin (OCN), was determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Protein expression of the odontogenic-related marker dentine sialoprotein (DSP) and the osteogenic-related marker RUNX2 was measured by Western blotting analysis. One-way analysis of variance (anova) and Student's t-test were used for statistical analysis (a = 0.05). RESULTS: The overexpression of CREB enhanced mineralized nodule formation and up-regulated (P < 0.05) the mRNA levels of odonto/osteogenic-related markers, including ALP, Col I, RUNX2, OSX and OCN, and also increased (P < 0.05) the protein expression of DSP and RUNX2. In contrast, the silencing of CREB inhibited (P < 0.05) the mineralization capacity of the SCAPs and decreased (P < 0.05) the expression of odonto/osteogenic-related markers. CONCLUSION: Up-regulation of CREB expression promoted o...