Telocytes in gastric lamina propria of the Chinese giant salamander, Andrias davidianus

作者：Hui Zhang, Shengwei Zhong, Pengcheng Yu, Tingting Ge, Shasha Peng, Xiaoquan Guo, Zuohong Zhou · 发表于：Scientific Reports · 年份：2016 · DOI：10.1038/srep33554 · 被引用次数：15 · 研究领域：Extracellular vesicles in disease、MicroRNA in disease regulation、RNA Interference and Gene Delivery

In this study, we attempt to identify gastric telocytes (TCs) of the Chinese giant salamander Andrias davidianus, by light microscopy, immunohistochemistry and transmission electron microscopy (TEM) methods. Toluidine blue staining showed TCs with one to two very thin and long telopodes (Tps) that were located in gastric lamina propria. Tps had characteristic structures, including podoms, podomers and dichotomous branching. Immunohistochemistry showed the existence of CD34(+)/PDGFRα(+) TCs with moniliform Tps in stroma and were close to gastric glands and blood vessels. TEM micrographs also demonstrated the presence of TCs in interstitium between gastric glands. TCs/Tps were located in close proximity to gastric glands, blood vessels, endocrine cells and stem cells. In particular, Tps frequently surrounded stem cells. TCs and Tps, Tps and stem cells established close contacts. Moreover, the exosomes were also found near TCs/Tps. Our data confirmed the presence of TCs in gastric lamina propria of the amphibian, and suggested that TCs cooperate with resident stem cells to regulate endocrine cells and gastric glands regeneration and homeostasis.