Human pluripotent stem cell-derived acinar/ductal organoids generate human pancreas upon orthotopic transplantation and allow disease modelling

作者：Meike Hohwieler, Anett Illing, Patrick Hermann, T. Mayer, Marianne Stockmann, Lukas Perkhofer, Tim Eiseler, Justin S. Antony, Martin C. Müller, Susanne Renz, Chao‐Chung Kuo, Qiong Lin, Matthias Sendler, Markus Breunig, Susanne Kleiderman, André Lechel, Martin Zenker, Michael Leichsenring, Jonas Rosendahl, Martin Zenke, Bruno Sáinz, Julia Mayerle, Ivan G. Costa, Thomas Seufferlein, Michael Kormann, Martin Wagner, Stefan Liebau, Alexander Kleger · 发表于：Gut · 年份：2016 · DOI：10.1136/gutjnl-2016-312423 · 被引用次数：213 · 研究领域：Pluripotent Stem Cells Research、Pancreatic function and diabetes、Cystic Fibrosis Research Advances

Objective The generation of acinar and ductal cells from human pluripotent stem cells (PSCs) is a poorly studied process, although various diseases arise from this compartment. Design We designed a straightforward approach to direct human PSCs towards pancreatic organoids resembling acinar and ductal progeny. Results Extensive phenotyping of the organoids not only shows the appropriate marker profile but also ultrastructural, global gene expression and functional hallmarks of the human pancreas in the dish. Upon orthotopic transplantation into immunodeficient mice, these organoids form normal pancreatic ducts and acinar tissue resembling fetal human pancreas without evidence of tumour formation or transformation. Finally, we implemented this unique phenotyping tool as a model to study the pancreatic facets of cystic fibrosis (CF). For the first time, we provide evidence that in vitro , but also in our xenograft transplantation assay, pancreatic commitment occurs generally unhindered in CF. Importantly, cystic fibrosis transmembrane conductance regulator (CFTR) activation in mutated pancreatic organoids not only mirrors the CF phenotype in functional assays but also at a global expression level. We also conducted a scalable proof-of-concept screen in CF pancreatic organoids using a set of CFTR correctors and activators, and established an mRNA-mediated gene therapy approach in CF organoids. Conclusions Taken together, our platform provides novel opportunities to model pancreat...