The Multivesicular Bodies (MVBs)-Localized AAA ATPase LRD6-6 Inhibits Immunity and Cell Death Likely through Regulating MVBs-Mediated Vesicular Trafficking in Rice

作者：Xiaobo Zhu, Junjie Yin, Sihui Liang, Ruihong Liang, Xiaogang Zhou, Zhixiong Chen, Wen Zhao, Jing Wang, Weitao Li, Min He, Can Yuan, Koji Miyamoto, Bingtian Ma, Jichun Wang, Peng Qin, Weilan Chen, Yuping Wang, Wenming Wang, Xianjun Wu, Hisakazu Yamane, Lihuang Zhu, Shigui Li, Xuewei Chen · 发表于：PLoS Genetics · 年份：2016 · DOI：10.1371/journal.pgen.1006311 · 被引用次数：78 · 研究领域：Plant-Microbe Interactions and Immunity、Legume Nitrogen Fixing Symbiosis、Plant Pathogenic Bacteria Studies

Previous studies have shown that multivesicular bodies (MVBs)/endosomes-mediated vesicular trafficking may play key roles in plant immunity and cell death. However, the molecular regulation is poorly understood in rice. Here we report the identification and characterization of a MVBs-localized AAA ATPase LRD6-6 in rice. Disruption of LRD6-6 leads to enhanced immunity and cell death in rice. The ATPase activity and homo-dimerization of LRD6-6 is essential for its regulation on plant immunity and cell death. An ATPase inactive mutation (LRD6-6E315Q) leads to dominant-negative inhibition in plants. The LRD6-6 protein co-localizes with the MVBs marker protein RabF1/ARA6 and interacts with ESCRT-III components OsSNF7 and OsVPS2. Further analysis reveals that LRD6-6 is required for MVBs-mediated vesicular trafficking and inhibits the biosynthesis of antimicrobial compounds. Collectively, our study shows that the AAA ATPase LRD6-6 inhibits plant immunity and cell death most likely through modulating MVBs-mediated vesicular trafficking in rice.