miR-378 attenuates muscle regeneration by delaying satellite cell activation and differentiation in mice

作者：Ping Zeng, Wanhong Han, Changyin Li, Hu Li, Dahai Zhu, Yong Zhang, Xiaohong Liu · 发表于：Acta Biochimica et Biophysica Sinica · 年份：2016 · DOI：10.1093/abbs/gmw077 · 被引用次数：26 · 研究领域：Muscle Physiology and Disorders、Tissue Engineering and Regenerative Medicine、Mesenchymal stem cell research

Skeletal muscle mass and homeostasis during postnatal muscle development and regeneration largely depend on adult muscle stem cells (satellite cells). We recently showed that global overexpression of miR-378 significantly reduced skeletal muscle mass in mice. In the current study, we used miR-378 transgenic (Tg) mice to assess the in vivo functional effects of miR-378 on skeletal muscle growth and regeneration. Cross-sectional analysis of skeletal muscle tissues showed that the number and size of myofibers were significantly lower in miR-378 Tg mice than in wild-type mice. Attenuated cardiotoxin-induced muscle regeneration in miR-378 Tg mice was found to be associated with delayed satellite cell activation and differentiation. Mechanistically, miR-378 was found to directly target Igf1r in muscle cells both in vitro and in vivo These miR-378 Tg mice may provide a model for investigating the physiological and pathological roles of skeletal muscle in muscle-associated diseases in humans, particularly in sarcopenia.