Oxysterol-binding protein-related protein 4L promotes cell proliferation by sustaining intracellular Ca2+ homeostasis in cervical carcinoma cell lines

作者：Jiwei Li, Yan-Lin Xiao, Chaofeng Lai, Ning Lou, Hongling Ma, Biying Zhu, Wenbin Zhong, Daoguang Yan · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.11671 · 被引用次数：24 · 研究领域：Signaling Pathways in Disease、Galectins and Cancer Biology、Ion Channels and Receptors

// Ji-Wei Li 1, 2, * , Yan-Lin Xiao 1, 2, * , Chao-Feng Lai 1, 2, * , Ning Lou 3 , Hong-Ling Ma 1, 2 , Bi-Ying Zhu 1, 2 , Wen-Bin Zhong 1, 2 , Dao-Guang Yan 1, 2 1 Department of Biotechnology, Jinan University, Guangzhou, 510632, China 2 The Key Laboratory of Functional Protein Research of Guangdong Higher Education Institutes, Jinan University, Guangzhou, 510632, China 3 State Key Laboratory of Oncology in Southern China, Collaborative Innovation Center of Cancer Medicine, Guangzhou, 510060, China * These authors have contributed equally to this work Correspondence to: Dao-Guang Yan, email: tydg@jnu.edu.cn Wen-Bin Zhong, email: winbeyz@163.com Keywords: ORP4L, Ca 2+ homeostasis, NFAT, IP 3 R1, proliferation Received: February 22, 2016     Accepted: August 09, 2016     Published: August 29, 2016 ABSTRACT Oxsterol binding protein-related protein 4 (ORP4) is essential for cell proliferation, but the underlying mechanism is unclear. ORP4 is expressed as three variants, ORP4L, ORP4M and ORP4S. Here, we reported that silencing of ORP4L with specific small interfering RNA (siRNA) inhibited the proliferation of human cervical cancer cell lines C33A, HeLa and CaSki, the reverse effect being observed in ORP4L overexpressing cells. For molecular insight, we found that ORP4L maintained intracellular Ca 2+ homeostasis. Through this mechanism, ORP4L activated nuclear factor of activated T cells (NFAT) activity and thus promoted expression of a gene ...