Tissue-Engineered Human Psoriatic Skin Supplemented with Cytokines as anin VitroModel to Study Plaque Psoriasis

作者：Claudia Pouliot-Bérubé, Karine Zaniolo, Sylvain L. Guérin, Roxane Pouliot · 发表于：Regenerative Medicine · 年份：2016 · DOI：10.2217/rme-2016-0037 · 被引用次数：25 · 研究领域：Psoriasis: Treatment and Pathogenesis、Spondyloarthritis Studies and Treatments、Advancements in Transdermal Drug Delivery

AIM: Psoriasis is a chronic inflammatory skin disease. To study its complex etiology, a psoriatic skin substitute model supplemented with a cytokine cocktail has been used. MATERIALS & METHODS: Reconstructed psoriatic skin substitutes were supplemented with a cocktail of four cytokines: TNF-α, IL-1α, IL-6 and IL-17A, to monitor their impact on gene expression by DNA microarray. RESULTS: Gene profiling analyses identified several deregulated genes reported as being also deregulated in psoriasis skin in vivo (S100A12, IL-8, DEFB4A and KYNU). The expression of those genes was dramatically increased compared with basal levels of controls (p < 0.005 to < 0.05). CONCLUSION: Psoriatic substitutes supplemented with a cocktail of TNF-α, IL-1α, IL-6 and IL-17A showed similar transcriptome alterations to those found in psoriasis.