Boar seminal plasma exosomes maintain sperm function by infiltrating into the sperm membrane

作者：Jianbin Du, Jian Shen, Yuanxian Wang, Chuanying Pan, Weijun Pang, Hua Diao, Wuzi Dong · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.11315 · 被引用次数：182 · 研究领域：Sperm and Testicular Function、Extracellular vesicles in disease、Genital Health and Disease

// Jian Du 1 , Jian Shen 1 , Yuanxian Wang 1 , Chuanying Pan 1 , Weijun Pang 1 , Hua Diao 2 and Wuzi Dong 1 1 College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi, P. R. China 2 NPFPC Key Laboratory of Contraceptives and Devices, Shanghai Institute of Planned Parenthood Research (SIPPR), Institutes of Reproduction and Development, Fudan University, Shanghai, P. R. China Correspondence to: Wuzi Dong, email: // Keywords : seminal plasma exosomes; boar sperm quality; capacitation; liquid storage; Pathology Section Received : May 20, 2016 Accepted : July 28, 2016 Published : August 16, 2016 Abstract Seminal plasma ingredients are important for maintenance of sperm viability. This study focuses on the effect of boar seminal plasma exosomes on sperm function during long-term liquid storage. Boar seminal plasma exosomes had typical nano-structure morphology as measured by scanning electron microscopy (SEM) and molecular markers such as AWN, CD9 and CD63 by western blot analysis. The effect on sperm parameters of adding different ratio of boar seminal plasma exosomes to boar sperm preparations was analyzed. Compared to the diluent without exosomes, the diluent with four times or sixteen times exosomes compared to original semen had higher sperm motility, prolonged effective survival time, improved sperm plasma membrane integrity ( p < 0.05), increased total antioxidant capacity (T-AOC) activity and decreased malondialdehyde (MDA) cont...