ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes

作者：Aleksandra Dakic, Kyle A. DiVito, Shuang Fang, Frank A. Suprynowicz, Anirudh Gaur, Xin Li, Nancy Palechor-Ceron, Vera Simić, Sujata Maiti Choudhury, Songtao Yu, Cynthia M. Simbulan‐Rosenthal, Dean S. Rosenthal, Richard Schlegel, Xuefeng Liu · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.11458 · 被引用次数：44 · 研究领域：Cancer-related Molecular Pathways、Cell death mechanisms and regulation、Hippo pathway signaling and YAP/TAZ

// Aleksandra Dakic 1, 2 , Kyle DiVito 3 , Shuang Fang 1, 2 , Frank Suprynowicz 1, 2 , Anirudh Gaur 3 , Xin Li 4 , Nancy Palechor-Ceron 1, 2 , Vera Simic 1, 2 , Sujata Choudhury 1, 2 , Songtao Yu 1, 2 , Cynthia M. Simbulan-Rosenthal 3 , Dean Rosenthal 3 , Richard Schlegel 1, 2 , Xuefeng Liu 1, 2 1 Department of Pathology, Georgetown University Medical School, Washington, DC 20057, USA 2 Center for Cell Reprogramming, Georgetown University Medical School, Washington, DC 20057, USA 3 Department of Molecular and Cell Biology and Biochemistry, Georgetown University Medical School, Washington, DC 20057, USA 4 Department of Biostatistics, Bioinformatics, Georgetown University Medical School, Washington, DC 20057, USA Correspondence to: Xuefeng Liu, email: xuefeng.liu@georgetown.edu Keywords: Myc, ROCK, cytoskeleton, apoptosis, telomerase Received: May 04, 2016      Accepted: August 11, 2016      Published: August 20, 2016 ABSTRACT The Myc/Max/Mad network plays a critical role in cell proliferation, differentiation and apoptosis and c-Myc is overexpressed in many cancers, including HPV-positive cervical cancer cell lines. Despite the tolerance of cervical cancer keratinocytes to high Myc expression, we found that the solitary transduction of the Myc gene into primary cervical and foreskin keratinocytes induced rapid cell death. These findings suggested that the anti-apoptotic activity of E7 in cervical cancer cells might be responsib...