The novel raw starch-digesting amylase obtained from Chalara paradoxa. Part 2. Determination of optimum culture conditions for the Chalara enzyme production.

作者：Hiroshi Ishigami, Hitoshi Hashimoto, Keiji Kainuma · 发表于：Journal of the Japanese Society of Starch Science · 年份：1985 · DOI：10.5458/jag1972.32.189 · 被引用次数：15 · 研究领域：Enzyme Production and Characterization、Phytase and its Applications、Enzyme Catalysis and Immobilization

The culture conditions for the production of a new raw starch-digesting amylase which had been discovered in Chalara paradoxa were studied. Optimum culture medium consisted of 4.0% raw sago starch, 6.5% corn steep liquor, 0.7% beef extract, 0.3% NaCl and 0.08% FeSO4⋅7H2O. Optimum initial pH and optimum temperature were 4.5 and 30°C, respectively. This enzyme had higher affinity to raw starch compared with previously reported raw starch digesting amylases and hydrolyzed raw starch to glucose.