Relieving effect of Artemisia argyi aqueous extract on immune stress in broilers

作者：P. F. Zhang, Binlin Shi, Junling Su, Yunshuang Yue, Zhuang Cao, W. B. Chu, K. Li, Sumei Yan · 发表于：Journal of Animal Physiology and Animal Nutrition · 年份：2016 · DOI：10.1111/jpn.12553 · 被引用次数：43 · 研究领域：Animal Nutrition and Physiology、Pharmacological Effects of Natural Compounds、Essential Oils and Antimicrobial Activity

This experiment aimed to investigate the relieving action of Artemisia argyi aqueous extract (AAE) on immune stress in broiler chickens. A 2 × 2 factorial design was used to test the effect of 2 dietary treatments (adding 0 or 1000 mg/kg AAE) and 2 immune stress treatments (injecting saline or lipopolysaccharide (LPS)). A total of 96 one-day-old Arbor Acres (AA) broilers were randomly divided into four treatment groups with six replicates, four birds in each replicate. Broilers in Treatment groups 1 and 2 were fed with the basal diet, and those in Treatment groups 3 and 4 were fed with the experimental diet supplemented with 1000 mg/kg AAE. On days 14, 16, 18 and 20, broilers in both Treatments 1 and 3 were injected intra-abdominally with LPS solution at the dose of 500 μg LPS per kg BW with the LPS dissolved in sterile saline at a concentration of 100 μg/ml, and those in Treatments 2 and 4 were injected intra-abdominally with equal amount of sterile 0.9% saline. Blood samples were collected on days 21 and 28. The results showed that dietary supplementation of AAE prevented reductions in average daily gain (ADG) and average daily feed intake (ADFI) of broilers caused by LPS on d 15-21. On day 21, the injection of LPS increased serum adrenocorticotropic hormone (ACTH) and corticosterone (CORT); meanwhile, feeding AAE reduced the rise of CORT caused by LPS. Immune parameters such as interleukin-1 (IL-1), interleukin-2 (IL-2), interleukin-6 (IL-6), immunoglobulin G (IgG) and imm...