Detection ofListeria monocytogenesin CSF from Three Patients with Meningoencephalitis by Next-Generation Sequencing

作者：Ming Yao, Jiali Zhou, Yi‐Cheng Zhu, Yinxin Zhang, Xia Lv, Ruixue Sun, Ao Shen, Haitao Ren, Liying Cui, Hongzhi Guan, Honglong Wu · 发表于：Journal of Clinical Neurology · 年份：2016 · DOI：10.3988/jcn.2016.12.4.446 · 被引用次数：68 · 研究领域：Listeria monocytogenes in Food Safety、Salmonella and Campylobacter epidemiology、Biosensors and Analytical Detection

BACKGROUND AND PURPOSE: Encephalitis caused by Listeria monocytogenes (L. monocytogenes) is rare but sometimes fatal. Early diagnosis is difficult using routine cerebrospinal fluid (CSF) tests, while next-generation sequencing (NGS) is increasingly being used for the detection and characterization of pathogens. METHODS: This study set up and applied unbiased NGS to detect L. monocytogenes in CSF collected from three cases of clinically suspected listeria meningoencephalitis. RESULTS: Three cases of patients with acute/subacute meningoencephalitis are reported. Magnetic resonance imaging and blood cultures led to a suspected diagnosis of L. monocytogenes, while the CSF cultures were negative. Unbiased NGS of CSF identified and sequenced reads corresponding to L. monocytogenes in all three cases. CONCLUSIONS: This is the first report highlighting the feasibility of applying NGS of CSF as a diagnostic method for central nervous system (CNS) L. monocytogenes infection. Routine application of this technology in clinical microbiology will significantly improve diagnostic methods for CNS infectious diseases.