Purification of a Specific Placental Plasminogen Activator Inhibitor by Monoclonal Antibody and Its Complex Formation with Plasminogen Activator

作者：Birger Åstedt, Ingegerd Lecander, Thomas Brodin, Arne Lundblad, Karin Löw · 发表于：Thrombosis and Haemostasis · 年份：1985 · DOI：10.1055/s-0038-1661250 · 被引用次数：159 · 研究领域：Blood Coagulation and Thrombosis Mechanisms、Hemophilia Treatment and Research、Protease and Inhibitor Mechanisms

A monoclonal antibody of IgG2a-type was obtained against a specific fast acting plasminogen activator inhibitor found in placenta. The placental inhibitor was purified by affinity chromatography using the monoclonal antibody and additionally in a FPLC-system. A strong complex formation was found between the inhibitor and urokinase and also with the two-chain form of plasminogen activator of the tissue-type. A weaker complex was found between the placental inhibitor and the one-chain form of the tissue-type activator.