Evidence for four classes of microtubules in individual cells

作者：O. Behnke, Arthur Forer · 发表于：Journal of Cell Science · 年份：1967 · DOI：10.1242/jcs.2.2.169 · 被引用次数：423 · 研究领域：Microtubule and mitosis dynamics、Reproductive Biology and Fertility、Genetic and Kidney Cyst Diseases

ABSTRACT Experiments were performed on crane-fly spermatids (Nephrotoma suturalis Loew), ratsperm, and rat tracheal cilia to test whether all microtubules respond in the same way to different treatments. Crane-fly spermatids contain cytoplasmic microtubules, accessory tubules, and the 9 + 2 complex of tubules ; rat sperm and rat tracheal cilia contain only the 9 + 2 tubules. Crane-fly spermatid tubules responded to the experimental treatments as follows. After colchicine treatment, or storage at 0°C, the cytoplasmic microtubules disappeared, while the 9 + 2 tubules were normal. After storage at 50 °C the cytoplasmic microtubules disappeared, and then the 9 + 2 tubules were affected : first the central tubules and B-tubules were affected, and later the A-tubules. After brief pepsin treatment, the 9 doublet tubules disappeared, while the other tubules appeared normal ; after prolonged pepsin treatment the accessory, central, and cytoplasmic tubules disappeared. After negative staining at pH 7, the cytoplasmic microtubules were never seen, the central tubules were only sometimes seen, the B-tubules were sometimes fragmented, and the A-tubules were intact. On the basis of these responses, it was concluded that there are 4 classes of tubules in crane-fly spermatids, namely cytoplasmic microtubules; accessory tubules and central tubules (of the 9 + 2 complex) ; B-tubules (of the 9 + 2 complex) ; and A-tubules (of the 9 + 2 complex). At least some of the different responses appeared...