A New Selective Agent for Eukaryotic Cloning Vectors

作者：J. K. Davies, Antonio Jiménez · 发表于：American Journal of Tropical Medicine and Hygiene · 年份：1980 · DOI：10.4269/ajtmh.1980.29.1089 · 被引用次数：129 · 研究领域：Plant Disease Resistance and Genetics、Insect and Pesticide Research、Plant-Microbe Interactions and Immunity

An aminoglycoside antibiotic, G418, has been shown to be an inhibitor of many pro- and eukaryotes at concentrations from 1-300 microgram/ml. A bacterial R-plasmid determinant that phosphorylates and inactivates antibiotic G418 can be introduced into yeast by transformation and expresses resistance to G418. It is suggested that this combination of antibiotic and dominant resistance mechanism may be useful in recombinant DNA studies as a cloning selection in eukaryotes.