miR-200b is a key regulator of tumor progression and metabolism targeting lactate dehydrogenase A in human malignant glioma

作者：Su Hu, Qian Jiang, Dongdong Luo, Lei Zhao, Xin Fu, Yuqin Chen, Xue Song, Lihua Li, Hailin Zhao, Yingfang He, Biao Peng · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.10301 · 被引用次数：38 · 研究领域：MicroRNA in disease regulation、Cancer, Hypoxia, and Metabolism、Glioma Diagnosis and Treatment

// Su Hu 1, * , Qian Jiang 2, * , Dongdong Luo 1 , Lei Zhao 3 , Xin Fu 2 , Yuqin Chen 2 , Xue Song 4 , Lihua Li 1 , Hailin Zhao 1 , Yingfang He 1 , Biao Peng 1 1 Department of Neurosurgery, Cancer Center of Guangzhou Medical University, Guangzhou, Guangdong, 510095, China 2 State Key Laboratory of Respiratory Disease, The 1st Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, 510120, China 3 Department of Physiology, School of Basic Science, Guangzhou Medical University, Guangzhou, Guangdong, 510182, China 4 Guangdong Provincial Hospital of TCM Breast Department, Guangzhou, Guangdong, 510006, China * These authors have contributed equally to this work Correspondence to: Biao Peng, email: pengbiaopengbiao@msn.com Keywords: glioma, miR-200b, LDHA, proliferation, metabolism Received: December 04, 2015 Accepted: June 04, 2016 Published: June 27, 2016 ABSTRACT Lactate dehydrogenase A (LDHA) is involved in various cancers. In this study, we investigated the expression and function of LDHA in glioma. We found that LDHA was up-regulated in glioma samples. Furthermore, we found that overexpression of LDHA promoted proliferation, invasion and glycolysis in glioma cells. Luciferase reporter assays confirmed that LDHA was a direct target of miR-200b. miR-200b was found to be down-regulated in glioma samples, which was inversely correlated with LDHA expression. Repression of LDHA by miR-200b suppressed the glycolysis, cell proliferation and invasion of glioma cells...