ISL1, a novel regulator of CCNB1 , CCNB2 and c-MYC genes, promotes gastric cancer cell proliferation and tumor growth

作者：Qiong Shi, Weiping Wang, Zhuqing Jia, Ping Chen, Kangtao Ma, Chunyan Zhou · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.9269 · 被引用次数：76 · 研究领域：RNA modifications and cancer、Glycosylation and Glycoproteins Research、Ubiquitin and proteasome pathways

// Qiong Shi 1, * , Weiping Wang 1, * , Zhuqing Jia 1 , Ping Chen 1 , Kangtao Ma 1 , Chunyan Zhou 1 1 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education of China, Peking University, Beijing, P.R. China * These authors contributed equally to this work Correspondence to: Chunyan Zhou, email: chunyanzhou@bjmu.edu.cn Keywords: ISL1, gastric cancer, proliferation, CCNB, c-MYC Received: October 05, 2015      Accepted: April 22, 2016      Published: May 10, 2016 ABSTRACT I slet-1 ( ISL1 ) belongs to the LIM homeodomain transcription factor family, which is specifically expressed in certain tissue types only. Previously, we reported that ISL1 is aberrantly overexpressed in gastric cancer (GC). However, its role in GC is not clear. Here, we report that ISL1 is aberrantly upregulated not only in human gastric carcinoma tissues but also in some GC cell lines. Upregulated ISL1 expression enhanced xenografted gastric carcinoma development, while ISL1 knockdown inhibited GC growth in nude mice. ISL1 overexpression promoted GC cell proliferation, colony formation, and cell growth in soft agar, and facilitated cell cycle transition in GC cells, demonstrated an increase in the proportion of cells in the G 2 /M and S phases and a decrease in the proportio...