Immunoproteomics of Aspergillus for the development of biomarkers and immunotherapies

作者：Olaf Kniemeyer, Frank Ebel, Thomas Krüger, Petra Bächer, Alexander Scheffold, Ting Luo, Maria Straßburger, Axel A. Brakhage · 发表于：PROTEOMICS - CLINICAL APPLICATIONS · 年份：2016 · DOI：10.1002/prca.201600053 · 被引用次数：27 · 研究领域：Antifungal resistance and susceptibility、Fungal Infections and Studies、Mycotoxins in Agriculture and Food

Filamentous fungi of the genus Aspergillus play significant roles as pathogens causing superficial and invasive infections as well as allergic reactions in humans. Particularly invasive mycoses caused by Aspergillus species are characterized by high mortality rates due to difficult diagnosis and insufficient antifungal therapy. The application of immunoproteomic approaches has a great potential to identify new targets for the diagnosis, therapy, and vaccine development of diseases caused by Aspergillus species. Serological proteome analyses (SERPA) that combine 2D electrophoresis with Western blotting are still one of the most popular techniques for the identification of antigenic proteins. However, recently a growing number of approaches have been developed to identify proteins, which either provoke an antibody response or which represent targets of T-cell immunity in patients with allergy or fungal infections. Here, we review advances in the studies of immune responses against pathogenic Aspergilli as well as the current status of diagnosis and immunotherapy of Aspergillus infections.