Genetic variation of carotenoids, vitamin E and phenolic compounds in Provitamin A biofortified maize

作者：Tawanda Muzhingi, Natalia Palacios‐Rojas, Alejandra Miranda, María Luisa Cabrera, Kyung‐Jin Yeum, Guangwen Tang · 发表于：Journal of the Science of Food and Agriculture · 年份：2016 · DOI：10.1002/jsfa.7798 · 被引用次数：71 · 研究领域：Antioxidant Activity and Oxidative Stress、Plant Reproductive Biology、Genomics, phytochemicals, and oxidative stress

Abstract BACKGROUND Biofortified maize is not only a good vehicle for provitamin A carotenoids for vitamin A deficient populations in developing countries but also a source of vitamin E, tocochromanols and phenolic compounds, which have antioxidant properties. Using high‐performance liquid chromatography and a total antioxidant performance assay, the present study analyzed the antioxidant variation and antioxidant activity of 36 provitamin A improved maize hybrids and one common yellow maize hybrid. RESULTS The ranges of major carotenoids in provitamin A carotenoids biofortified maize were zeaxanthin [1.2–13.2 µg g−1 dry weight (DW)], β‐cryptoxanthin (1.3–8.8 µg g−1 DW) and β‐carotene (1.3–8.0 µg g−1 DW). The ranges of vitamin E compounds identified in provitamin A carotenoids biofortified maize were α‐tocopherol (3.4–34.3 µg g−1 DW), γ‐tocopherol (5.9–54.4 µg g−1 DW), α‐tocotrienol (2.6–19.5 µg g−1 DW) and γ‐tocotrienol (45.4 µg g−1 DW). The ranges of phenolic compounds were γ‐oryzanol (0.0–0.8 mg g−1 DW), ferulic acid (0.4–3.6 mg g−1 DW) and p‐coumaric acid (0.1–0.45 mg g−1 DW). There was significant correlation between α‐tocopherol and cis isomers of β‐carotene (P < 0.01). Tocotrienols were correlated with α‐tocopherol and γ‐oryzanol (P < 0.01). CONCLUSION Genotype was significant in determining the variation in β‐cryptoxanthin, β‐carotene, α‐tocopherol and γ‐tocopherol contents (P < 0.01). A genotype × environment interaction was observed for γ‐tocopherol content (P < 0.0...