The behaviour of fibroblasts migrating from chick heart explants: changes in adhesion, locomotion and growth, and in the distribution of actomyosin and fibronectin

作者：John Couchman, David Allan Rees · 发表于：Journal of Cell Science · 年份：1979 · DOI：10.1242/jcs.39.1.149 · 被引用次数：244 · 研究领域：Polymer Surface Interaction Studies、Cell Adhesion Molecules Research、Tissue Engineering and Regenerative Medicine

Fibroblasts migrating from heart explants of chick embryos at first have a high rate of locomotion but lack focal contacts or adhesions and also lack substantial actin-containing bundles. A meshwork of 7-nm filaments is present particularly in submembranous regions and is proposed to be directed towards efficient locomotion whilst maintaining a high degree of spreading. Also during the first 48 h there is little production of extracellular fibronectin and the growth rate is low. Later, these fibroblasts develop focal contacts and focal adhesions together with actomyosin bundles, with a parallel increase in fibronectin expression. We propose that progressive immobilization by the development of focal adhesions and actomyosin structures occurs to set these cells up for growth.