Three‐Dimensional Control of DNA Hybridization by Orthogonal Two‐Color Two‐Photon Uncaging

作者：Manuela A. H. Fichte, Xenia M. M. Weyel, Stephan Junek, Florian Schäfer, Cyril Herbivo, Maurice Goeldner, Alexandre Specht, Josef Wachtveitl, Alexander Heckel · 发表于：Angewandte Chemie International Edition · 年份：2016 · DOI：10.1002/anie.201603281 · 被引用次数：71 · 研究领域：Photochromic and Fluorescence Chemistry、Photoreceptor and optogenetics research、Retinal Development and Disorders

We successfully introduced two-photon-sensitive photolabile groups ([7-(diethylamino)coumarin-4-yl]methyl and p-dialkylaminonitrobiphenyl) into DNA strands and demonstrated their suitability for three-dimensional photorelease. To visualize the uncaging, we used a fluorescence readout based on double-strand displacement in a hydrogel and in neurons. Orthogonal two-photon uncaging of the two cages is possible, thus enabling complex scenarios of three-dimensional control of hybridization with light.