Development of gene‐based markers for the Turnip mosaic virus resistance gene retr02 in Brassica rapa

作者：Guoliang Li, Wei Qian, Shujiang Zhang, Shi-fan Zhang, Fei Li, Hui Zhang, Jian Wu, Xiaowu Wang, Rifei Sun · 发表于：Plant Breeding · 年份：2016 · DOI：10.1111/pbr.12372 · 被引用次数：20 · 研究领域：Plant Virus Research Studies、Plant Disease Resistance and Genetics、Plant Pathogenic Bacteria Studies

Abstract Turnip mosaic virus (Tu MV ) is responsible for a serious disease that affects the production of Chinese cabbage. Previous studies have cloned a series of Tu MV resistance genes and developed molecular markers. In this study, a derived cleaved amplified polymorphism sequence ( dCAPS ) marker and a Kompetitive Allele Specific PCR ( KASP ) marker were developed based on a single recessive gene, retr02 , which confers broad‐spectrum Tu MV resistance in Chinese cabbage by means of an additional G at the junction of exon 1 and intron 1. The two markers were able to detect the retr02 allele in Chinese cabbage accessions used in breeding programmes. Compared with the dCAPS marker, the KASP marker was flexible, cost‐effective and quick to process, which is likely to be beneficial in establishing high‐throughput assays for marker‐assisted selection.