Aptamer-Functionalized Fluorescent Silica Nanoparticles for Highly Sensitive Detection of Leukemia Cells

作者：Juntao Tan, Nuo Yang, Zixi Hu, Jing Su, Jian‐Hong Zhong, Yang Yang, Yating Yu, Jianmeng Zhu, Dabin Xue, Yingying Huang, Yingying Huang, Zongqiang Lai, Yong Huang, Yong Huang, Xiaoling Lü, Yongxiang Zhao · 发表于：Nanoscale Research Letters · 年份：2016 · DOI：10.1186/s11671-016-1512-8 · 被引用次数：61 · 研究领域：Advanced biosensing and bioanalysis techniques、Biosensors and Analytical Detection、RNA Interference and Gene Delivery

A simple, highly sensitive method to detect leukemia cells has been developed based on aptamer-modified fluorescent silica nanoparticles (FSNPs). In this strategy, the amine-labeled Sgc8 aptamer was conjugated to carboxyl-modified FSNPs via amide coupling between amino and carboxyl groups. Sensitivity and specificity of Sgc8-FSNPs were assessed using flow cytometry and fluorescence microscopy. These results showed that Sgc8-FSNPs detected leukemia cells with high sensitivity and specificity. Aptamer-modified FSNPs hold promise for sensitive and specific detection of leukemia cells. Changing the aptamer may allow the FSNPs to detect other types of cancer cells.