Macrotiter assay for coronavirus-neutralizing activity in cats using a canine continuous cell line (A-72).

作者：Barlough Je, Jacobson Rh, Scott Fw · 发表于：PubMed · 年份：1983 · 被引用次数：7 · 研究领域：Animal Virus Infections Studies、Virus-based gene therapy research、Viral gastroenteritis research and epidemiology

A heterologous neutralization assay for feline infectious peritonitis virus serology was developed using a single continuous cell line of canine origin, A-72, which is susceptible to cytopathic infection with both transmissible gastroenteritis virus of pigs and canine coronavirus. Of several coronavirus isolates tested, the 1-71 isolate of canine coronavirus demonstrated the most effective neutralization by serum and body fluids of cats with histopathologically confirmed feline infectious peritonitis.