Intestinal pathway of internalisation of lactic acid bacteria and gut mucosal immunostimulation.

作者：Gabriela Perdigón, Marcela Medina, Elisa Vintiñi, JC Valdez · 发表于：PubMed · 年份：2003 · 被引用次数：61 · 研究领域：Probiotics and Fermented Foods、Infant Nutrition and Health、Pediatric health and respiratory diseases

cells of labelled single strains of Lactobacillus casei, Lactobacillus acidophilus, Lactobacillus delbrueckii ssp. bulgaricus and Streptococcus thermophilus. For electron microscopy studies unlabelled bacteria were used. Histological preparations of the small and large intestine were performed 1 hour after administration of the bacteria. Fluorescent bacteria of L. casei and S. thermophilus were found only in Peyer's patches while L. delbrueckii ssp. bulgaricus and L. acidophilus were observed in Peyer's patches and in the small intestine. L. acidophilus was also found in the large intestine. We confirmed these findings by electron microscopy. We determined that for L. casei the pathway of internalisation was via the M and FAE cells of Peyer's patches, while for S. thermophilus, L. acidophilus and L. delbrueckii ssp. bulgaricus the interaction with the immune cells of Peyer's patches was through the follicle associated epithelium (FAE). L. delbrueckii ssp. bulgaricus and L. acidophilus also interacted with the epithelial cells of the small intestine and L. acidophilus with epithelial cells of the large intestine. These results suggest that the different effects of LAB on the mucosal immunostimulation, are related to the different pathways of gut internalisation used to take contact with the immune cells associated with the lamina propria intestinal.