Down-regulation of Krüppel-like factor-4 by microRNA-135a-5p promotes proliferation and metastasis in hepatocellular carcinoma by transforming growth factor-β1

作者：Shanshan Yao, Chuan Tian, Youcheng Ding, Qingwang Ye, Yong Gao, Ning Yang, Qi Li · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.9934 · 被引用次数：36 · 研究领域：Kruppel-like factors research、MicroRNA in disease regulation、Cancer-related molecular mechanisms research

// Shanshan Yao 1, * , Chuan Tian 1, * , Youcheng Ding 2 , Qingwang Ye 3 , Yong Gao 1 , Ning Yang 3 , Qi Li 1, 4 1 Department of Oncology, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120,China 2 Department of General Surgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, 200120,China 3 Department of Liver Surgery, Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Shanghai, 200438, China 4 Department of Oncology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China * These authors have contributed equally to this work Correspondence to: Qi Li, email: Leeqi2001@hotmail.com Ning Yang, email: Lancet00@163.com Keywords: KLF4, miR-135a-5p, TGF-β1, hepatocellular carcinoma Received: October 17, 2015     Accepted: May 11, 2016     Published: June 10, 2016 ABSTRACT Krüppel-like Factor-4 (KLF4) is a zinc finger transcription factor which plays an important role in cell cycle, proliferation and apoptosis. In Hepatocellular Carcinoma (HCC), the function of KLF4 has been characterized as tumor suppressor. However, the mechanism remains largely unknown. In this study, we demonstrated that TGF-β1 down-regulated KLF4 by activating miR-135a-5p. MiR-135a-5p promoted proliferation and metastasis in HCC cells by direct targeting KLF4 both in vitro and in vivo. In addition, miR-135a-5p expression was up-regulated ...