On the structure of cytolipin R, a ceramide tetrahexoside hapten from rat lymphosarcoma

作者：Roger A. Laine, Charles C. Sweeley, Yongsheng Li, Alemka Kisić, Maurice M. Rapport · 发表于：Journal of Lipid Research · 年份：1972 · DOI：10.1016/s0022-2275(20)39386-x · 被引用次数：54 · 研究领域：Sphingolipid Metabolism and Signaling、Carbohydrate Chemistry and Synthesis、Lipid Membrane Structure and Behavior

Cytolipin R, a ceramide tetrahexoside isolated from rat lymphosarcoma, was studied by sequential hydrolysis with specific glycosidases which revealed the anomeric configurations of the glycosidic bonds. Sugar linkages were established by combined gas-liquid chromatography and mass spectrometry of the partially methylated alditol acetates prepared after permethylation and hydrolysis of the intact lipid. Results indicated the structure of cytolipin R to be N-acetylgalactosaminyl(beta1-->3)galactosyl(alpha1-->3) galactosyl(beta1-->4)glucosyl ceramide. Cytolipin K (globoside I) differs in having a -galactosyl(alpha1-->4)galactosyl- internal linkage, and this difference must account for the immunological differences between cytolipin K and cytolipin R.