[The comparison of contents of uracil, xanthine and hypoxanthine in five species of leech].

作者：L Liu, Rui Jin, Guangdi Xu · 发表于：PubMed · 年份：1999 · 被引用次数：2 · 研究领域：Leech Biology and Applications

Three constituents(uracil, xanthine, hypoxanthine) were isolated from five species of leech and determined by reversed phase HPLC. The column employed was Shim-pack CLC-ODS C18(150 mm x 6 mm). The mobile phase was 0.05 mol/l ammonium phophate dibasic solution(pH = 8.4). The flow rate was 0.8 ml/min and detection was effected at 254 nm. This method is accurate, rapid and reproducible. Analytical data for five species and Whitmania pigra samples from different places were given.