A simple enzymic method for the synthesis of adenosine 5′-[α-32P]triphosphate on a preparative scale

作者：Bronwen Martin, H. Paul Voorheis · 发表于：Biochemical Journal · 年份：1977 · DOI：10.1042/bj1610555 · 被引用次数：25 · 研究领域：Biochemical and Molecular Research、RNA and protein synthesis mechanisms、Peptidase Inhibition and Analysis

A simple, rapid and inexpensive method is described for the enzymic synthesis of [alpha-32P]ATP from [32P]Pi on a preparative scale with an overall yield of 53%. The final product contained all of the detectable radioactivity (less than 99.9%) in the alpha position and has been shown to behave identically with commerically availabe [alpha-32P]ATP during the synthesis of 3':5'-cyclic AMP in the reaction catalysed by adenylate cyclase.