Estrogenic effects of flavonoid components in Xiaoyao powder

作者：Jiun-Hong Chen, Naimin Zhang, Yuting Wang, Jinglei Wang, Seung Hyeon Ji, Wenjun Dang, Shaoping Li, Lin Feng · 发表于：Genetics and Molecular Research · 年份：2016 · DOI：10.4238/gmr.15017500 · 被引用次数：14 · 研究领域：Estrogen and related hormone effects、Natural product bioactivities and synthesis、Medicinal Plant Pharmacodynamics Research

The objective of this study was to evaluate the estrogenic effects and mechanisms of three flavonoid components in Xiaoyao powder: quercetin, kaempferol, and isorhamnetin. The drugs were used to treat estrogen receptor (ER)-positive human breast cancer MCF-7 cells, and proliferation was measured using the MTT method. The expression of proteins and mRNA of the ER subtype were measured using western blotting and real time polymerase chain reaction. The quercetin (10(-2) μM, 10(-3) μM), kaempferol (100 μM, 10(-2) μM), and isorhamnetin (10(-3) μM) promoted the proliferation of MCF-7 cells, and the expression of ERα and ERβ proteins and mRNA were all increased significantly (P < 0.05). These effects were reversed by treatment with 0.1 μM estrogen antagonist ICI182780. Three flavonoid components in Xiaoyao powder increased the expression of proteins and mRNA of ERα and ERβ and promoted the proliferation of MCF-7 cells. These estrogenic effects were mediated by the ER.