Free Amino Acid Patterns of Certain Tissues From Potassium and/or Protein-Deficient Rats

作者：Michael Iacobellis, Edward Muntwyler, Charles L. Dodgen · 发表于：American Journal of Physiology-Legacy Content · 年份：1956 · DOI：10.1152/ajplegacy.1956.185.2.275 · 被引用次数：52 · 研究领域：Ion Transport and Channel Regulation、Muscle metabolism and nutrition、Child Nutrition and Feeding Issues

Tracings of amino acid patterns obtained chromatographically on the tungstic acid filtrates of skeletal muscle, diaphragm, kidney and liver from normal, and protein- and/or K-deficient rats have been presented. Also presented were the tracings of amino acid patterns of the same tissues from rats maintained on a control and a low-K diet and administered DCA. Evidence was presented indicating that the amino acids lysine and arginine were increased in the skeletal muscle, diaphragm and kidney from rats with K deficiency, with or without DCA administration. Concomitant to these changes, there appeared to be a decreased concentration of the amino acids aspartic acid and glutamic acid. The amino acid pattern of the liver from K-deficient rats remained essentially normal. The administration of a KCl solution by stomach tube during 24 hours to the K-deficient rats resulted in a normalization of the amino acid patterns. The amino acid patterns of skeletal muscle, diaphragm and kidney from protein- and K-deficient rats were found to be normal. However, the amino acid patterns of these tissues were found to have reverted to those observed with the K-depleted rats upon supplementing the diet of the doubly deficient animals with protein for 6 days. The significance of a possible transfer of amino acids along with a shift of H+ into muscle cells in exchange for K+ in the development of an alkalosis found in K-deficient rats was briefly commented upon.