THE MOLECULAR TRANSFORMATIONS OF ACTIN

作者：Wilfried F. H. M. Mommaerts · 发表于：Journal of Biological Chemistry · 年份：1952 · DOI：10.1016/s0021-9258(18)55600-3 · 被引用次数：81 · 研究领域：Cellular Mechanics and Interactions

In Papers I and II, a description was given of globular and fibrous actin and of the polymerization process which converts the former into the latter.It was established by Straub and Feuer (14), Laki, Bowen, and Clark (4), and Mommaerts (7) that a nucleotide which was considered identical with ATPl plays a rale in the reaction between actin molecules.In the present investigation, the reaction between actin and ATP has been studied in detail. EXPERIMENTAL MethodsPure globular and fibrous actins were prepared as described previously, and the same analytical procedures were employed (10,11).Chromatographic identification of adenosine polyphosphates was performed according to Cohn and Carter (2).Phosphate determinations were done by the method of Berenblum and Chain (1).Occasionally, the Fiske and Subbarow method was used, although for most phases of this work its sensitivity was too low. Results