Effect of silymarin on N-nitrosodiethylamine induced hepatocarcinogenesis in rats.

作者：Gopalakrishnan Ramakrishnan, Titto Alby Augustine, S Jagan, Radhakrishnan Vinodhkumar, Thiruvengadam Devaki · 发表于：PubMed · 年份：2007 · 被引用次数：105 · 研究领域：Silymarin and Mushroom Poisoning、Flavonoids in Medical Research、Melamine detection and toxicity

AIM: To study the effect of silymarin on the levels of tumor markers and MDA (malondialdehyde)-DNA adduct formation during N-nitrosodiethylamine induced hepatocellular carcinoma in male Wistar albino rats. METHODS: The levels of AFP, CEA and activities of liver marker enzymes in serum, MDA-DNA immunohistochemistry were done according to standard procedures in the control and experimental groups of rats. RESULTS: Hepatocellular carcinoma was evidenced from significant (p < 0.05) increases of alpha-fetoprotein, carcinoembryonic antigen, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, acid phosphatase, lactate dehydrogenase, gamma-glutamyltransferase and 5'-nucleotidase in serum and increased MDA-DNA adducts were also observed in the tissue sections of hepatocellular carcinoma. Silymarin treatment significantly attenuated the alteration of these markers and decreased the levels of MDA-DNA adduct formation. CONCLUSION: Silymarin could be developed as a promising chemotherapeutic adjuvant for the treatment of liver cancer.