Separation of glycosyl diglycerides from phosphatides using silicic acid column chromatography

作者：Marie L. Vorbeck, G.V. Marinetti · 发表于：Journal of Lipid Research · 年份：1965 · DOI：10.1016/s0022-2275(20)39630-9 · 被引用次数：244 · 研究领域：Enzyme Catalysis and Immobilization、Microbial Metabolic Engineering and Bioproduction、Diet, Metabolism, and Disease

A procedure has been developed for the separation of the glycosyl diglycerides from the phosphatides of Gram-positive bacteria on columns of silicic acid. The method utilizes mixtures of acetone in chloroform for elution of the glycosyl diglycerides, followed by increasing amounts of methanol in chloroform for elution of the phosphatides. The course of the fractionation was followed by means of phosphorus and carbohydrate determinations and by paper chromatography. The completeness of the separation of the phosphatides from the sugar-containing lipids was shown also by chromatographing a total lipid extract containing P32-labeled phosphatides.