Periodic acid/Schiff staining of glycoproteins immobilized on a blotting matrix.

作者：Mats Strömqvist, H Gruffman · 发表于：PubMed · 年份：1992 · 被引用次数：40 · 研究领域：Glycosylation and Glycoproteins Research、RNA and protein synthesis mechanisms、Machine Learning in Bioinformatics

A simple and sensitive method for the detection of glycoproteins and glycopeptides in solution and in polyacrylamide gels is described. This method combines the well-known periodic acid/Schiff stain with protein blotting. Compared with direct staining of a polyacrylamide gel, the sensitivity is considerably increased. Using a set of glycoproteins, we have found the sensitivity to be about 4 ng carbohydrate.