[Expression of human interleukin-12 in Baculovirus expression system].

作者：Linqi Yang, X Zhang, Xiaoqin Long, Xuchuang Chen, X Wang, Li Y · 发表于：PubMed · 年份：2001 · 研究领域：Viral Infectious Diseases and Gene Expression in Insects

Human Interleukin-12 (hIL-12) is a key immunoregulatory cytokine and the only heterodimeric cytokine found by far. It is a disulfide-linked heterodimer composed of a 35 kD light chain (P35) and a 40 kD heavy chain (P40). Two recombinant transfer vector plasmids pAcAB3-p35 (including hIL-12 p35 gene) and pAcAB3-p40 (including hIL-12 p40 gene) were constructed and co-transfected the insect cells Sf9 with AcNPV BaculoGold Linearized Baculovirus DNA. The obtained recombinant viruses, AcNPV-OCC(-)-hiL-12 (p35) and AcNPV-OCC(-)-hIL-12 (p40), were used to infect the Sf9 cells separately, and the supernatants of culture and cell lysate were examined by using SDS-PAGE and Western blot. Results showed that both hIL-12 p35 and p40 gene were successfully expressed in insect cells and secreted. The expression productions had immunogenicity.