Cytoplasmic RNA sequences complementary to cloned chick δ-crystallin cDNA show size heterogeneity.

作者：D. J. Bower, L. H. Errington, N. Wainwright, Christina Sime, Sidney M. Morris, R.M. Clayton · 发表于：Biochemical Journal · 年份：1982 · DOI：10.1042/bj2010339 · 被引用次数：40 · 研究领域：Connexins and lens biology、RNA regulation and disease、Molecular Biology Techniques and Applications

Double-stranded complementary DNA (cDNA) sequences were prepared from day-old chick lens total polysomal RNA and inserted into the unique PstI restriction site of the plasmid pBR322. Colonies containing sequences complementary to abundant lens poly(A)-containing RNA sequences were identified by using lens 32P-labelled cDNA. Some of these clones have been characterized as containing delta-crystallin mRNA coding sequences by genomic DNA blot hybridization and RNA blot hybridizations. Hybridization of labelled DNA from such clones to RNA blots detected four size classes of delta-crystallin RNA sequences, although Southern blots indicated that there are probably only two delta-crystallin genes.