Effects of live yeast supplementation on lipopolysaccharide-induced inflammatory responses in broilers

作者：Weiwei Wang, Zhui Li, Wanli Ren, Yunshuang Yue, Yuming Guo · 发表于：Poultry Science · 年份：2016 · DOI：10.3382/ps/pew191 · 被引用次数：34 · 研究领域：Animal Nutrition and Physiology、Meat and Animal Product Quality、Aquaculture disease management and microbiota

The effects of supplemental live yeast (LY) on inflammatory responses in broilers challenged with lipopolysaccharide (LPS) were investigated. One-day-old broilers were randomly divided into two treatment groups with two subgroups of each (8 replicate pens; 10 birds/pen) and were fed a basal diet without or with 0.5 g/kg of LY (Saccharomyces cerevisiae NCYC 47Hr+, 1.0 × 1010 CFU/g). Birds from each subgroup of the two treatment groups were intra-abdominally injected with LPS (1.5 mg/kg of BW) or saline at 21, 23, 25, and 27 d of age. Samples were obtained after 8 h of the first injection (d 21) and the last injection (d 27), respectively. Results showed that no treatment differences (P > 0.05) were detected in the relative spleen and bursa weights, as well as serum lysozyme activity and ceruloplasmin content regardless of the immunological status. LY addition tended to alleviate (P = 0.097) LPS-induced increase in serum α-acid glycoprotein content on d 27. LPS induced increased (P < 0.05) serum nitric oxide content and myeloperoxidase activity on d 21 and 27, however, there was a tendency towards reduced (P < 0.10) serum nitric oxide content and myeloperoxidase activity on d 21 in response to LY inclusion. Besides, LY-fed birds had lower (P < 0.05) serum nitric oxide content on d 27 relative to the control counterparts. LPS resulted in increased (P < 0.05) relative mRNA expression of splenic interleukin-1β on d 21 and 27, but which was lower (P < 0.05) in LY-treated birds comp...