Targeted next generation sequencing of endoscopic ultrasound acquired cytology from ampullary and pancreatic adenocarcinoma has the potential to aid patient stratification for optimal therapy selection

作者：Ferga C. Gleeson, Sarah E. Kerr, Benjamin R. Kipp, Jesse S. Voss, Douglas M. Minot, Zheng Jin Tu, Michael R. Henry, Rondell Patrell Graham, George Vasmatzis, John C. Cheville, Konstantinos N. Lazaridis, Michael J. Levy · 发表于：Oncotarget · 年份：2016 · DOI：10.18632/oncotarget.9440 · 被引用次数：98 · 研究领域：Pancreatic and Hepatic Oncology Research、Pancreatitis Pathology and Treatment、Neuroendocrine Tumor Research Advances

// Ferga C. Gleeson 1 , Sarah E. Kerr 2 , Benjamin R. Kipp 2 , Jesse S. Voss 2 , Douglas M. Minot 2 , Zheng Jin Tu 3 , Michael R. Henry 2 , Rondell P. Graham 2 , George Vasmatzis 4 , John C. Cheville 4 , Konstantinos N. Lazaridis 1, 4 , Michael J. Levy 1 1 Division of Gastroenterology & Hepatology, Mayo Clinic Rochester, MN, USA 2 Department of Laboratory Medicine & Pathology, Mayo Clinic Rochester, MN, USA 3 Division of Biomedical Statics & Informatics, Department of Health Sciences Research, Mayo Clinic Rochester, MN, USA 4 Center for Individualized Medicine, Mayo Clinic, Rochester, MN, USA Correspondence to: Ferga C. Gleeson, email: gleeson.ferga@mayo.edu Keywords: endoscopic ultrasound fine needle aspiration, pancreatic adenocarcinoma, targeted next-generation sequencing, mutation concordance, personalized medicine Received: April 04, 2016 Accepted: April 24, 2016 Published: May 18, 2016 ABSTRACT Background & Aims: Less than 10% of registered drug intervention trials for pancreatic ductal adenocarcinoma (PDAC) include a biomarker stratification strategy. The ability to identify distinct mutation subsets via endoscopic ultrasound fine needle aspiration (EUS FNA) molecular cytology could greatly aid clinical trial patient stratification and offer predictive markers. We identified chemotherapy treatment naïve ampullary adenocarcinoma and PDAC patients who underwent EUS FNA to assess multigene mutational frequency and diversity with a surgical resection concordance assessment...