Determination of Lysozyme by Graphene Oxide–Polyethylene Glycol-Based Fluorescence Resonance Energy Transfer

作者：Yali Xie, Jianxin An, Pengzhi Shi, Nengsheng Ye · 发表于：Analytical Letters · 年份：2016 · DOI：10.1080/00032719.2016.1172232 · 被引用次数：14 · 研究领域：Advanced biosensing and bioanalysis techniques、Graphene and Nanomaterials Applications、Carbon and Quantum Dots Applications

The surface of graphene oxide was modified by bis(3-aminopropyl)-terminated polyethylene glycol to produce a composite graphene–polyethylene glycol. The graphene oxide/polyethylene glycol maximum absorption peak in the ultraviolet–visible spectrum was redshifted, and transmission electron microscope images showed that graphene oxide was cleaved into small nanosheets to form graphene oxide/polyethylene glycol. The dispersibility of graphene oxide/polyethylene glycol in physiological solution was higher than for graphene oxide. The optimum composite of graphene oxide/polyethylene glycol was used as a quencher in a fluorescence resonance energy transfer aptasensor for the determination of lysozyme detection. The results showed that graphene oxide/polyethylene glycol rapidly and efficiently quenched the fluorescence of the dye-labeled aptamer. The fluorescence was recovered by adding lysozyme to the system. The aptamer fluorescence intensity exhibited a strong linear dependence on the lysozyme concentration from 50 to 300 nM, and the lysozyme detection limit was approximately 11 nM. This method was used for the determination of lysozyme in egg whites, demonstrating that this approach is a promising alternative for the determination of lysozyme.